Difference between revisions of "Part:BBa K3081104"
| Line 4: | Line 4: | ||
This part is composed of a 42-nt-long dCas9 handle hairpin for Cas9 protein binding and a 40-nt-long transcription terminator hairpin derived from S. pyogenes. When designing a sgRNA for CRISPR/Cas9 system, you just need to put the target sequence before the sgRNA scaffold. | This part is composed of a 42-nt-long dCas9 handle hairpin for Cas9 protein binding and a 40-nt-long transcription terminator hairpin derived from S. pyogenes. When designing a sgRNA for CRISPR/Cas9 system, you just need to put the target sequence before the sgRNA scaffold. | ||
| − | https://2019.igem.org/wiki/images/ | + | https://2019.igem.org/wiki/images/2/27/T--Peking--pbad.png |
<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here | ||
Revision as of 19:00, 20 October 2019
scaffold of sgRNA in CRISPR/Cas system
This part is composed of a 42-nt-long dCas9 handle hairpin for Cas9 protein binding and a 40-nt-long transcription terminator hairpin derived from S. pyogenes. When designing a sgRNA for CRISPR/Cas9 system, you just need to put the target sequence before the sgRNA scaffold.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]