Difference between revisions of "Part:BBa K2957077"

Revision as of 18:55, 20 October 2019

pL1 position 1 backbone (ST1-2)

Contents: pL1 backbone, with Amp resistance What is it?: A Basic part; in this particular case we call it a pL0 (through Modular Cloning Golden Gate Type II Assembly) What does it do?: It is a backbone for the composite parts we made. It was important for our project in particular because of its resistance and was necessary to make any composite parts. It has an Ampicillin resistance. How to use it?: You can use this part for Type IIS Cloning. It has BsaI cut sites that allows for this. With other Basic parts like pL0-P (promoter/regulatory sites), pL0-T (terminator part), pL0-5.2/pL0-3 (Inert pieces that form the UTR regions around the genetic circuit), you can use this part to make a composite part. So the pL0's are placed in a given backbone depending on the kind of basic part it is. In this case, we have the backbone part. See more on the Parts Overview Page or MIT iGEM 2019 wiki: <a href=https://2019.igem.org/Team:MIT/Parts>.

Sequence and Features

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal prefix found at 2434
Plasmid lacks a suffix.
Illegal SpeI site found at 167
12
INCOMPATIBLE WITH RFC[12]
Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal EcoRI site found at 2434
Illegal SpeI site found at 167
Illegal NotI site found at 2440
21
INCOMPATIBLE WITH RFC[21]
Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal EcoRI site found at 2434
Illegal BamHI site found at 174
23
INCOMPATIBLE WITH RFC[23]
Illegal prefix found at 2434
Plasmid lacks a suffix.
Illegal SpeI site found at 167
25
INCOMPATIBLE WITH RFC[25]
Illegal prefix found at 2434
Plasmid lacks a suffix.
Illegal XbaI site found at 2449
Illegal SpeI site found at 167
Illegal NgoMIV site found at 475
Illegal AgeI site found at 2303
1000
INCOMPATIBLE WITH RFC[1000]
Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal BsaI site found at 272
Illegal BsaI.rc site found at 2367
Illegal SapI site found at 2351
Illegal SapI.rc site found at 287

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K2957077 short</partinfo>
-Contents: pL1 backbone, with Amp resistance and RFP
+Contents: pL1 backbone, with Amp resistance
-What is it?: A Basic part; in this particular case we call it a pL0 (through Modular Cloning Golden Gate Type II Assembly) What does it do?: It is a backbone for the composite parts we made. It was important for our project in particular because of its resistance and was necessary to make any composite parts. It has an Ampicillin resistance and Red Fluorescence Protein (RFP).
+What is it?: A Basic part; in this particular case we call it a pL0 (through Modular Cloning Golden Gate Type II Assembly) What does it do?: It is a backbone for the composite parts we made. It was important for our project in particular because of its resistance and was necessary to make any composite parts. It has an Ampicillin resistance.
 How to use it?: You can use this part for Type IIS Cloning. It has BsaI cut sites that allows for this. With other Basic parts like pL0-P (promoter/regulatory sites), pL0-T (terminator part), pL0-5.2/pL0-3 (Inert pieces that form the UTR regions around the genetic circuit), you can use this part to make a composite part. So the pL0's are placed in a given backbone depending on the kind of basic part it is. In this case, we have the backbone part. See more on the Parts Overview Page or MIT iGEM 2019 wiki: <a href=https://2019.igem.org/Team:MIT/Parts>.