Difference between revisions of "Part:BBa K3113302"

Revision as of 18:55, 20 October 2019

pCAG_Gag-HiBiT-L7Ae

This plasmid codes for the coat protein of gag fused to a split luciferase fused to an RNA binding protein. This construct forms virus-like particles which can be detected via a split luciferase assay. Through the RNA binding protein, the vesicles can be loaded with RNA.

Usage

pCAG_GAG-HiBit-L7Ae construct is a Composite part that has been designed to achieve VLP formation inside HEK293T/MIN6-K8 cells and enable the transport of chosen RNA information inside vesicles through L7Ae-C/D box interaction (together with construct BBa_K3113301^[1] and BBa_K3113303^[2]).

Biology

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 3019
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 2319

[1] ttps://parts.igem.org/Part:BBa_K3113301

[2] ttps://parts.igem.org/Part:BBa_K3113303

[1]

[2]

@@ Line 7: / Line 7: @@
 ===Usage===
-pCAG_GAG-HiBit-L7Ae construct is a Composite part that has been designed to achieve VLP formation inside HEK293T/MIN6-K8 cells and enable the transport of chosen RNA information inside vesicles through L7Ae-C/D box interaction (together with construct BBa_K3113301<ref>https://parts.igem.org/Part:BBa_K3113301</ref> and BBa_K3113203<ref>https://parts.igem.org/Part:BBa_K3113303</ref>).
+pCAG_GAG-HiBit-L7Ae construct is a Composite part that has been designed to achieve VLP formation inside HEK293T/MIN6-K8 cells and enable the transport of chosen RNA information inside vesicles through L7Ae-C/D box interaction (together with construct BBa_K3113301<ref>https://parts.igem.org/Part:BBa_K3113301</ref> and BBa_K3113303<ref>https://parts.igem.org/Part:BBa_K3113303</ref>).
 ===Biology===