Difference between revisions of "Part:BBa K3171181"

Revision as of 18:11, 20 October 2019

HisD gRNA 2

We experimented with CRISPR with the aim to make a histidine auxotroph by knocking out HisD in both E. coli and V. natriegens. To make a histidine auxotroph by knocking out HisD in E. coli specific gRNA targets were selected for knockout of the HisD gene. We designed 3 homologous parts Part:BBa_K3171177, Part:BBa_K3171178, Part:BBa_K3171179 in order to decide on the correct homologous part required for knockout. 3 gRNA target sites Part:BBa_K3171180, Part:BBa_K3171181, Part:BBa_K3171182 were selected for knock out to determine the exact region for knockout.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

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 <partinfo>BBa_K3171181 short</partinfo>
-We experimented with CRISPR with the aim to make a histidine auxotroph by knocking out HisD in both E. coli and V. natriegens. Specific gRNA targets were selected for knockout of the HisD gene. We designed 3 homologous parts (BBa_K3171177, BBa_K3171178, BBa_K3171179) and 3 gRNA target sites (BBa_K3171180, BBa_K3171181, BBa_K3171182) which all fall under the category of basic parts.
+We experimented with CRISPR with the aim to make a histidine auxotroph by knocking out HisD in both E. coli and V. natriegens. To make a histidine auxotroph by knocking out HisD in E. coli specific gRNA targets were selected for knockout of the HisD gene. We designed 3 homologous parts [[Part:BBa_K3171177]], [[Part:BBa_K3171178]], [[Part:BBa_K3171179]] in order to decide on the correct homologous part required for knockout. 3 gRNA target sites [[Part:BBa_K3171180]], [[Part:BBa_K3171181]], [[Part:BBa_K3171182]] were selected for knock out to determine the exact region for knockout.
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