Difference between revisions of "Part:BBa K2978000"

Revision as of 17:58, 20 October 2019

Causes cell lysis of the pathogen Clostridium difficile

This part makes reference to an endolysin from a bacteriophage that infects Clostridium difficile. It is a segment of the complete protein CD27L, which residues from 2 to 143 are homolougous to the domain N-acetyl-muramoyl-L-alanine amidase. This part codes for residues 1 to 179 of the mentioned protein. It has been codon optimized for E.coli. In previous works this domain has shown a faster lysis to different strains of the pathogen and a high level of selectivity against clostridia compared to the complete protein (Wang et al., 2015).

Usage

The expression of this part is under a T7 promoter, therefore the chasis must contain the T7 polymerase. In our lab we confirmed that E. coli BL21 de3 produces the lysin when IPTG is applied, as this strain has the respective polymerase under pLac promoter.

Characterization

Sequence and Features

Sequence and Features