Difference between revisions of "Part:BBa K3171178"
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<partinfo>BBa_K3171178 short</partinfo> | <partinfo>BBa_K3171178 short</partinfo> | ||
| − | We experimented with CRISPR with the aim to make a histidine auxotroph by knocking out HisD in both E. coli and V. natriegens. | + | We experimented with CRISPR with the aim to make a histidine auxotroph by knocking out HisD in both E. coli and V. natriegens. To make a histidine auxotroph by knocking out HisD in E. coli specific gRNA targets were selected for knockout of the HisD gene. We designed 3 homologous parts [[Part:BBa_K3171177]], [[Part:BBa_K3171178]], [[Part:BBa_K3171179]] in order to decide on the correct homologous part required for knockout. 3 gRNA target sites [[Part:BBa_K3171180]], [[Part:BBa_K3171181]], [[Part:BBa_K3171182]] were selected for knock out to determine the exact region for knockout. |
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Revision as of 17:57, 20 October 2019
HisD 1500bp Homologous part 2
We experimented with CRISPR with the aim to make a histidine auxotroph by knocking out HisD in both E. coli and V. natriegens. To make a histidine auxotroph by knocking out HisD in E. coli specific gRNA targets were selected for knockout of the HisD gene. We designed 3 homologous parts Part:BBa_K3171177, Part:BBa_K3171178, Part:BBa_K3171179 in order to decide on the correct homologous part required for knockout. 3 gRNA target sites Part:BBa_K3171180, Part:BBa_K3171181, Part:BBa_K3171182 were selected for knock out to determine the exact region for knockout.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 704
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 1117
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI site found at 39