Difference between revisions of "Part:BBa K3007021"
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[[File:T--QHFZ-China--BBa K3007001 2.png|400px|thumb|left|Figure 1. Working mechanism of the uric acid detection system in E. coli. ]]
 
[[File:T--QHFZ-China--BBa K3007001 2.png|400px|thumb|left|Figure 1. Working mechanism of the uric acid detection system in E. coli. ]]
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We used the process shown in Fig. 2 to test if the UA detection system works well.
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<!-- Add more about the biology of this part here
 
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Revision as of 17:54, 20 October 2019


Coding sequence of dsRed

This year, QHFZ-China designed a UA monitor system in E. coli (Fig. 1). Pc is a constitutive promoter, Pcp6 promoter, and it promotes the expression of HucR and YgfU. If the concentration of uric acid (UA) in environment is low, HucR will bind to hucO sequence (located in PhucR), which inhibits the expression of downstream reporter, dsRed or sfGFP. When extracellular UA is present, YgfU can transport UA into the cytoplasm, which leads HucR dissociates from hucO, and induces the fluorescent protein expression.

Figure 1. Working mechanism of the uric acid detection system in E. coli.


We used the process shown in Fig. 2 to test if the UA detection system works well.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 337
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 337
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 337
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 337
  • 1000
    COMPATIBLE WITH RFC[1000]