Difference between revisions of "Part:BBa K3007021"

Revision as of 17:52, 20 October 2019

Coding sequence of dsRed

This year, QHFZ-China designed a UA monitor system in E. coli (Fig. 1). Pc is a constitutive promoter, Pcp6 promoter, and it promotes the expression of HucR and YgfU. If the concentration of uric acid (UA) in environment is low, HucR will bind to hucO sequence (located in PhucR), which inhibits the expression of downstream reporter, dsRed or sfGFP. When extracellular UA is present, YgfU can transport UA into the cytoplasm, which leads HucR dissociates from hucO, and induces the fluorescent protein expression.

Figure 1. Working mechanism of the uric acid detection system in E. coli.

Sequence and Features

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal PstI site found at 337
12
INCOMPATIBLE WITH RFC[12]
Illegal PstI site found at 337
21
COMPATIBLE WITH RFC[21]
23
INCOMPATIBLE WITH RFC[23]
Illegal PstI site found at 337
25
INCOMPATIBLE WITH RFC[25]
Illegal PstI site found at 337
1000
COMPATIBLE WITH RFC[1000]

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 <partinfo>BBa_K3007021 short</partinfo>
-In our design, we used dsRed, which could express red fluorescence, as a reporter to report if uric acid presented qualitatively and quantitatively.
+This year, QHFZ-China designed a UA monitor system in E. coli (Fig. 1). Pc is a constitutive promoter, Pcp6 promoter, and it promotes the expression of HucR and YgfU. If the concentration of uric acid (UA) in environment is low, HucR will bind to hucO sequence (located in PhucR), which inhibits the expression of downstream reporter, dsRed or sfGFP. When extracellular UA is present, YgfU can transport UA into the cytoplasm, which leads HucR dissociates from hucO, and induces the fluorescent protein expression.
+<br>
+[[File:T--QHFZ-China--BBa K3007001 2.png|400px|thumb|left|Figure 1. Working mechanism of the uric acid detection system in E. coli. ]]
 <!-- Add more about the biology of this part here