Difference between revisions of "Part:BBa K3171177"

Revision as of 17:51, 20 October 2019

HisD 3000bp Homologous part 1

We experimented with CRISPR with the aim to make a histidine auxotroph by knocking out HisD in both E. coli and V. natriegens. To make a histidine auxotroph by knocking out HisD in E. coli specific gRNA targets were selected for knockout of the HisD gene. We designed 3 homologous parts (BBa_K3171177, BBa_K3171178, BBa_K3171179) in order to decide on the correct homologous part. 3 gRNA target sites (BBa_K3171180, BBa_K3171181, BBa_K3171182) were selected for knock out which.

Sequence and Features

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal XbaI site found at 689
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 1472
21
COMPATIBLE WITH RFC[21]
23
INCOMPATIBLE WITH RFC[23]
Illegal XbaI site found at 689
25
INCOMPATIBLE WITH RFC[25]
Illegal XbaI site found at 689
Illegal AgeI site found at 1885
Illegal AgeI site found at 2788
1000
INCOMPATIBLE WITH RFC[1000]
Illegal SapI site found at 807

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 <partinfo>BBa_K3171177 short</partinfo>
-To make a histidine auxotroph by knocking out HisD in E. coli. Specific gRNA targets were selected for knockout of the HisD gene. We designed 3 homologous parts (BBa_K3171177, BBa_K3171178, BBa_K3171179) in order to decide on the correct homologous part. 3 gRNA target sites (BBa_K3171180, BBa_K3171181, BBa_K3171182) which all fall under the category of basic parts.
+We experimented with CRISPR with the aim to make a histidine auxotroph by knocking out HisD in both E. coli and V. natriegens. To make a histidine auxotroph by knocking out HisD in E. coli specific gRNA targets were selected for knockout of the HisD gene. We designed 3 homologous parts (BBa_K3171177, BBa_K3171178, BBa_K3171179) in order to decide on the correct homologous part. 3 gRNA target sites (BBa_K3171180, BBa_K3171181, BBa_K3171182) were selected for knock out which.
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