Difference between revisions of "Part:BBa K3265026:Design"

 
Line 7: Line 7:
  
 
===Design Notes===
 
===Design Notes===
−
Has a stop codon after EutN, has a linker between gfp and EutN.
 
  
 +
<b> This part should be used with a low copy number plasmid backbone </b> <br>
  
 +
We used this backbone: https://parts.igem.org/Part:BBa_K3265035 <br>
 +
It is suitable for protein expression in <i> P. chlororaphis </i> and <i> E.coli </i> <br>
  
 +
We use the very weak RBS J61100 that seems to work fine with keeping base expression levels below. <br>
 +
Furthermore, we use the more tight araBAD promoter system instead of the LacI which is known to be leaky to get more tight control of the protein expression. <br>
  
  

Revision as of 17:39, 20 October 2019


EutSMN bacterial microcompartment


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1293
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1232
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1067
    Illegal AgeI site found at 3824
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 3212


Design Notes

This part should be used with a low copy number plasmid backbone

We used this backbone: https://parts.igem.org/Part:BBa_K3265035
It is suitable for protein expression in P. chlororaphis and E.coli

We use the very weak RBS J61100 that seems to work fine with keeping base expression levels below.
Furthermore, we use the more tight araBAD promoter system instead of the LacI which is known to be leaky to get more tight control of the protein expression.


Source

IDT, synthesized

References