Difference between revisions of "Part:BBa K3171171:Design"

Revision as of 17:20, 20 October 2019

Vibrio natriegens native P1 promoter

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal prefix found in sequence at 7
Illegal suffix found in sequence at 305
12
INCOMPATIBLE WITH RFC[12]
Illegal EcoRI site found at 7
Illegal SpeI site found at 306
Illegal PstI site found at 320
Illegal NotI site found at 13
Illegal NotI site found at 313
21
INCOMPATIBLE WITH RFC[21]
Illegal EcoRI site found at 7
23
INCOMPATIBLE WITH RFC[23]
Illegal prefix found in sequence at 7
Illegal suffix found in sequence at 306
25
INCOMPATIBLE WITH RFC[25]
Illegal prefix found in sequence at 7
Illegal XbaI site found at 22
Illegal SpeI site found at 306
Illegal PstI site found at 320
1000
COMPATIBLE WITH RFC[1000]

@@ Line 7: / Line 7: @@
 ===Design Notes===
-The strength of the  P1 promoter was measured in the P1-mCherry construct, which was designed by 3A assembly method. A synthetic promoter library was constructed by enhancing the function and inducibility. The screening was done based on fluorescence intensity measured. The best variation of the promoter was determined based on fold change in the fluorescence intensity and low basal level.
 ===Source===