Difference between revisions of "Part:BBa K2923017"
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| − | Our bacterial three-hybrid (BH3) construct (inspired by Berry and Hochschild, 2018) is composed of two fusion proteins with MS2 and PP7 coat | + | Our bacterial three-hybrid (BH3) construct (inspired by Berry and Hochschild, 2018) is composed of two fusion proteins with MS2 and PP7 coat proteins and RNA. This present part is composed of two MS2 (from Escherichia phage MS2) stem-loops. MS2 is able to be specifically recognized by MS2 coat protein for linked all the parts of our construct together. This part permits to create a new RNA sequence bordered by MS2 and PP7 stem-loops. |
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Latest revision as of 17:13, 20 October 2019
Two MS2 stem-loops
Our bacterial three-hybrid (BH3) construct (inspired by Berry and Hochschild, 2018) is composed of two fusion proteins with MS2 and PP7 coat proteins and RNA. This present part is composed of two MS2 (from Escherichia phage MS2) stem-loops. MS2 is able to be specifically recognized by MS2 coat protein for linked all the parts of our construct together. This part permits to create a new RNA sequence bordered by MS2 and PP7 stem-loops.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal XbaI site found at 37
Illegal PstI site found at 25
Illegal PstI site found at 64 - 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 25
Illegal PstI site found at 64 - 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal XbaI site found at 37
Illegal PstI site found at 25
Illegal PstI site found at 64 - 25INCOMPATIBLE WITH RFC[25]Illegal XbaI site found at 37
Illegal PstI site found at 25
Illegal PstI site found at 64 - 1000COMPATIBLE WITH RFC[1000]