Difference between revisions of "Part:BBa K2923016"
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<partinfo>BBa_K2923016 short</partinfo> | <partinfo>BBa_K2923016 short</partinfo> | ||
− | Our bacterial three-hybrid (BH3) construct (inspired by Berry and Hochschild, 2018) is composed of two fusion proteins with MS2 and PP7 coat protein and RNA. This present part is composed of two PP7 (Pseudomonas phage PP7) stem-loops. PP7 | + | Our bacterial three-hybrid (BH3) construct (inspired by Berry and Hochschild, 2018) is composed of two fusion proteins with MS2 and PP7 coat protein and RNA. This present part is composed of two PP7 (Pseudomonas phage PP7) stem-loops. PP7 stem loops are able to be specifically recognized by PP7 coat proteins for linked all the parts of our construct together. This part permits to create a new RNA sequence bordered by MS2 and PP7 stem-loops. |
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Latest revision as of 17:12, 20 October 2019
Two PP7 stem-loops
Our bacterial three-hybrid (BH3) construct (inspired by Berry and Hochschild, 2018) is composed of two fusion proteins with MS2 and PP7 coat protein and RNA. This present part is composed of two PP7 (Pseudomonas phage PP7) stem-loops. PP7 stem loops are able to be specifically recognized by PP7 coat proteins for linked all the parts of our construct together. This part permits to create a new RNA sequence bordered by MS2 and PP7 stem-loops.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]