Difference between revisions of "Part:BBa K3139014"

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__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_K3139014 short</partinfo>
 
<partinfo>BBa_K3139014 short</partinfo>
J23100-fusion protein-HasA-B0015-J23106-TEVp-HasA-B0010
 
 
Efficiency measurement device 1 of anti-plasmodium system  
 
Efficiency measurement device 1 of anti-plasmodium system  
  
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<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here
 
===Usage and Biology===
 
===Usage and Biology===
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In the anti-plasmodium system of NAU-2019, the amount of both TEV protease (<a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K3139012">BBa_K3139012</a>) and anti-plasmodium fusion protein (<a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K3139013">BBa_K3139013</a>) should be kept at a certain proportion to avoid a waste of cell resources.
 
In the anti-plasmodium system of NAU-2019, the amount of both TEV protease (<a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K3139012">BBa_K3139012</a>) and anti-plasmodium fusion protein (<a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K3139013">BBa_K3139013</a>) should be kept at a certain proportion to avoid a waste of cell resources.
 
If the TEV protease is excessive, it shall be wasted though the fusion proteins are cleaved completely. If the fusion protein is excessive, it cannot be cleaved completely with insufficient TEV protease.
 
If the TEV protease is excessive, it shall be wasted though the fusion proteins are cleaved completely. If the fusion protein is excessive, it cannot be cleaved completely with insufficient TEV protease.
 
By testing different promoter combinations of TEVp and fusion protein, we tried to find out the most economical combination which has the least waste and most complete cleavage with the help of mathematic model.  
 
By testing different promoter combinations of TEVp and fusion protein, we tried to find out the most economical combination which has the least waste and most complete cleavage with the help of mathematic model.  
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>

Revision as of 17:05, 20 October 2019


J23100-fusion protein-B0015-J23106-TEVp-TEVp site-HasA-B0010 Efficiency measurement device 1 of anti-plasmodium system


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal NheI site found at 2232
    Illegal NheI site found at 2255
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 444
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 2582
    Illegal SapI.rc site found at 2930