Difference between revisions of "Part:BBa K2923008"
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<partinfo>BBa_K2923008 short</partinfo> | <partinfo>BBa_K2923008 short</partinfo> | ||
| − | An aptazyme is an aptamer-ribozyme combination with intrinsic RNA cleavage activity. This aptazyme is specific for guanine and guanosine ligands. It is composed of 3 different parts: a ribozyme module, a communication module | + | An aptazyme is an aptamer-ribozyme combination with intrinsic RNA cleavage activity. This aptazyme is specific for guanine and guanosine ligands. It is composed of 3 different parts: a ribozyme module, a communication module and an aptamer module. This sequence corresponds to inactive aptazyme: the ribozyme module is mutated and the aptazyme lost self-cleavage capability. iGEM Strasbourg 2019 used it as a negative control for the part: <partinfo>BBa_K2923003</partinfo> |
[[Image: Gua active et inactive.jpg|300px|thumb|center|Figure: A ribozyme module is in black. The Shine-Dalgarno (SD) is in blue. The guanine-sensing xpt aptamer (light grey) is connected to the ribozyme via a communication module (in green). The figure is reproduced from [Stifel, J., Spöring, M., and Hartig, J.S. (2019). Expanding the toolbox of synthetic riboswitches with guanine-dependent aptazymes. Synthetic Biology 4.] | [[Image: Gua active et inactive.jpg|300px|thumb|center|Figure: A ribozyme module is in black. The Shine-Dalgarno (SD) is in blue. The guanine-sensing xpt aptamer (light grey) is connected to the ribozyme via a communication module (in green). The figure is reproduced from [Stifel, J., Spöring, M., and Hartig, J.S. (2019). Expanding the toolbox of synthetic riboswitches with guanine-dependent aptazymes. Synthetic Biology 4.] | ||
Latest revision as of 17:02, 20 October 2019
Guanine inactif aptazyme
An aptazyme is an aptamer-ribozyme combination with intrinsic RNA cleavage activity. This aptazyme is specific for guanine and guanosine ligands. It is composed of 3 different parts: a ribozyme module, a communication module and an aptamer module. This sequence corresponds to inactive aptazyme: the ribozyme module is mutated and the aptazyme lost self-cleavage capability. iGEM Strasbourg 2019 used it as a negative control for the part: BBa_K2923003
Figure: A ribozyme module is in black. The Shine-Dalgarno (SD) is in blue. The guanine-sensing xpt aptamer (light grey) is connected to the ribozyme via a communication module (in green). The figure is reproduced from [Stifel, J., Spöring, M., and Hartig, J.S. (2019). Expanding the toolbox of synthetic riboswitches with guanine-dependent aptazymes. Synthetic Biology 4.]
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]