Difference between revisions of "Part:BBa K3317067"
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RNA thermometer, by subjecting the cells to temperatures greater than 37 ℃ the expression of the proteins placed downstream is allowed, this sequence is an improvement of (<partinfo>K1140006</partinfo>) | RNA thermometer, by subjecting the cells to temperatures greater than 37 ℃ the expression of the proteins placed downstream is allowed, this sequence is an improvement of (<partinfo>K1140006</partinfo>) | ||
| − | < | + | <-- Add more about the biology of this part here |
===Usage and Biology=== | ===Usage and Biology=== | ||
Revision as of 16:54, 20 October 2019
37 C inducible RNA thermometer
RNA thermometer, by subjecting the cells to temperatures greater than 37 ℃ the expression of the proteins placed downstream is allowed, this sequence is an improvement of (BBa_K1140006)
<-- Add more about the biology of this part here
Usage and Biology
The primary objective was to perform an "in silico" design to reduce the range of operation of previously reported thermometers (28-37 ° C), obtaining a more precise range and allowing greater control of the system.
Tests were performed in conjunction with the (BBa_K1140006) thermometer from 25 to 40 ° C, measuring at 586/610 nm (excitation / emission for mCherry) and 660 nm to obtain optical density of the culture and normalize results, which showed that the new thermometer controlled the expression of the reporter in a more precise range, like the predicted theoretically. The "in silico" design allows the optimization of regulatory elements, such as RNA thermometers, reducing the number of experiments to a minimum.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 63
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]