Difference between revisions of "Part:BBa K2973015"
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This part represents a small fragment of the virus' genome. It is a small DNA fragment(280 bp), that does not encode for any functional protein or toxin. It's a small part of a sequence that encodes for a DNA polymerase of the virus and in alternative reading frames it encodes for envelope proteins of the virus, or for a specific surface antigen. | This part represents a small fragment of the virus' genome. It is a small DNA fragment(280 bp), that does not encode for any functional protein or toxin. It's a small part of a sequence that encodes for a DNA polymerase of the virus and in alternative reading frames it encodes for envelope proteins of the virus, or for a specific surface antigen. | ||
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===Usage and Biology=== | ===Usage and Biology=== | ||
This part serves as a DNA template for amplification reactions (biomarker). In our project, we successfuly amplified a specific region of the part using PCR and Recombinase Polymerase Amplification (RPA). | This part serves as a DNA template for amplification reactions (biomarker). In our project, we successfuly amplified a specific region of the part using PCR and Recombinase Polymerase Amplification (RPA). | ||
Revision as of 16:48, 20 October 2019
Hepatitis B Virus fragment (281-560bp)
Hepatitis B is a DNA virus that attacks the liver and can cause both acute and chronic disease. This part represents a small fragment of the virus' genome. It is a small DNA fragment(280 bp), that does not encode for any functional protein or toxin. It's a small part of a sequence that encodes for a DNA polymerase of the virus and in alternative reading frames it encodes for envelope proteins of the virus, or for a specific surface antigen.
Usage and Biology
This part serves as a DNA template for amplification reactions (biomarker). In our project, we successfuly amplified a specific region of the part using PCR and Recombinase Polymerase Amplification (RPA).
PCR Reaction
RPA reaction after cleanup of the amplified product
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 210
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]