Difference between revisions of "Part:BBa K2973015"

 
Line 8: Line 8:
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here
 
===Usage and Biology===
 
===Usage and Biology===
 +
This part serves as a DNA template for amplification reactions (biomarker). In our project, we successfuly amplified a specific region of the part using PCR and Recombinase Polymerase Amplification (RPA).
  
 +
PCR Reaction
 +
 +
<html>
 +
  <head>
 +
      <title>HTML img Tag</title>
 +
  </head>
 +
 +
  <body>
 +
      <img src="https://static.igem.org/mediawiki/parts/5/59/Thessaly_2019_HBV_PCR_Gel.jpeg" width="200"
 +
        height="300">
 +
  </body>
 +
</html>
 +
 +
RPA reaction after cleanup of the amplified product
 +
 +
<html>
 +
  <head>
 +
      <title>HTML img Tag</title>
 +
  </head>
 +
 +
  <body>
 +
      <img src="https://2019.igem.org/wiki/images/a/af/T--Thessaly--Results_Figure9.jpg" width="200"
 +
        height="300">
 +
  </body>
 +
</html>
 
<!-- -->
 
<!-- -->
 
<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>

Revision as of 16:48, 20 October 2019


Hepatitis B Virus fragment (281-560bp)

Hepatitis B is a DNA virus that attacks the liver and can cause both acute and chronic disease. This part represents a small fragment of the virus' genome. It is a small DNA fragment(280 bp), that does not encode for any functional protein or toxin. It's a small part of a sequence that encodes for a DNA polymerase of the virus and in alternative reading frames it encodes for envelope proteins of the virus, or for a specific surface antigen.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 210
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]