Difference between revisions of "Part:BBa K2924045"
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The RBS is the location in which a ribosome binds to the mRNA and initiates the translation. The following RBS can be used in Bacillus subtilis for translation of mRNA into protein. | The RBS is the location in which a ribosome binds to the mRNA and initiates the translation. The following RBS can be used in Bacillus subtilis for translation of mRNA into protein. | ||
| − | In our case the RBS was used to initiate the transcription of heterologous expressed casein and whey proteins in <i>Bacillus subtilis</i> ([https://parts.igem.org/Part:BBa_K2924033 BBa_K2924033], [https://parts.igem.org/Part:BBa_K2924052 BBa_K2924052], [https://parts.igem.org/Part:BBa_K2924054 BBa_K2924054]) . | + | In our case the RBS was used to initiate the transcription of heterologous expressed casein and whey proteins in <i>Bacillus subtilis</i> ([https://parts.igem.org/Part:BBa_K2924033 BBa_K2924033], [https://parts.igem.org/Part:BBa_K2924052 BBa_K2924052], [https://parts.igem.org/Part:BBa_K2924054 BBa_K2924054]). |
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Latest revision as of 16:46, 20 October 2019
RBS for bacillus subtilis
RBS for protein translation in B. subtilis
Usage and Biology
The RBS is the location in which a ribosome binds to the mRNA and initiates the translation. The following RBS can be used in Bacillus subtilis for translation of mRNA into protein. In our case the RBS was used to initiate the transcription of heterologous expressed casein and whey proteins in Bacillus subtilis (BBa_K2924033, BBa_K2924052, BBa_K2924054).
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]