Difference between revisions of "Part:BBa K3127999"

 
 
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<partinfo>BBa_K3127999 short</partinfo>
 
<partinfo>BBa_K3127999 short</partinfo>
  
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This part contains LasR and CcdA.
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This part contains LasR and CcdA.This part contains LasR and CcdA, which can produce CcdA protein with 30C6HSL.
  
  
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<partinfo>BBa_K3127999 parameters</partinfo>
 
<partinfo>BBa_K3127999 parameters</partinfo>
 
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===Characterization===
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<b>Figure 1/2</b> shows the trends of each bacteria in the LCBC-system. We got the data through the enzyme-labelled instrument and analyzed it to get the figure. The y-axis is the number of cells, and the x-axis is fluorescence intensity. There are two kinds of E.coli. One kind of E.coli carried sfGFP on the genome. The other kind of E.coli carried RFP on the genome. Cells were individually cultured in the LB medium for 12 hours before common inoculated in 12-well plates in LB medium in the first 13 hours. Figure 1 shows the different fluorescent intensity of E.coli under the light of 640-650 nm wavelength for the first 13 hours and the light of 460-465 nm wavelength for the next 12 hours.Figure2 shows the different fluorescent intensity of E.coli under the light of 640-650 nm wavelength for the first 13 hours and the light of 530-535 nm wavelength for the next 12 hours.
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<div style="display:flex;justify-content:space-around">[[File:T-SDU CHINA-BBaS1.png|300px|thumb|center|<b>Figure 6:</b> We can see that after 12 hours of blue light exposure, the number of E. coli bacteria carrying RFP dropped by half. ]][[File:T-SDU CHINA-BBaS2.png|300px|thumb|center|<b>Figure 7:</b> After 12 hours of green light exposure, the number of E.coli carrying GFP decreased by half.]]</div>
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<b>Figure 3/4</b> shows the change in the ratio of two bacteria in LCBC system. We used CLSM to observe and process data. The second row shows the ratio of the two bacteria which have been exposed to the light of 640-650 nm wavelength for 12 hours and then to the light of 460-465 nm wavelength for the same time.  The third row shows the proportion of the two bacteria which have been under the light of 640-650 nm wavelength for 12 hours and the light of 530-535 nm wavelength for another 12 hours.
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<div>[[File:T-SDU CHINA-BBaS33.png|500px|center|]]</div>
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<div>[[File:T-SDU CHINA-BBaS43.png|500px|center|]]</div>

Latest revision as of 16:44, 20 October 2019


This part contains LasR and CcdA.

This part contains LasR and CcdA.This part contains LasR and CcdA, which can produce CcdA protein with 30C6HSL.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 372
    Illegal AgeI site found at 569
  • 1000
    COMPATIBLE WITH RFC[1000]


Characterization

Figure 1/2 shows the trends of each bacteria in the LCBC-system. We got the data through the enzyme-labelled instrument and analyzed it to get the figure. The y-axis is the number of cells, and the x-axis is fluorescence intensity. There are two kinds of E.coli. One kind of E.coli carried sfGFP on the genome. The other kind of E.coli carried RFP on the genome. Cells were individually cultured in the LB medium for 12 hours before common inoculated in 12-well plates in LB medium in the first 13 hours. Figure 1 shows the different fluorescent intensity of E.coli under the light of 640-650 nm wavelength for the first 13 hours and the light of 460-465 nm wavelength for the next 12 hours.Figure2 shows the different fluorescent intensity of E.coli under the light of 640-650 nm wavelength for the first 13 hours and the light of 530-535 nm wavelength for the next 12 hours.

Figure 6: We can see that after 12 hours of blue light exposure, the number of E. coli bacteria carrying RFP dropped by half.
Figure 7: After 12 hours of green light exposure, the number of E.coli carrying GFP decreased by half.

Figure 3/4 shows the change in the ratio of two bacteria in LCBC system. We used CLSM to observe and process data. The second row shows the ratio of the two bacteria which have been exposed to the light of 640-650 nm wavelength for 12 hours and then to the light of 460-465 nm wavelength for the same time. The third row shows the proportion of the two bacteria which have been under the light of 640-650 nm wavelength for 12 hours and the light of 530-535 nm wavelength for another 12 hours.

T-SDU CHINA-BBaS33.png
T-SDU CHINA-BBaS43.png