Difference between revisions of "Part:BBa K3147004:Design"

(→‎Design note)
(→‎Design note)
 
Line 11: Line 11:
 
We have observed that this construction is weakly expressed. We advise future users to add a RiboJ insulator and a BCD2 element to improve its expression.
 
We have observed that this construction is weakly expressed. We advise future users to add a RiboJ insulator and a BCD2 element to improve its expression.
  
−
A double terminator controls the expression of this parts (BBa_B0015).  
+
A double terminator controls the expression of this parts [[Part:BBa_B0015]].  
  
 
This sequence was synthesized by IDT DNA for iGEM Headquarters.
 
This sequence was synthesized by IDT DNA for iGEM Headquarters.

Latest revision as of 16:30, 20 October 2019

mRFP1 fused to a TEV-cleavable ssrA tag


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 691
  • 1000
    COMPATIBLE WITH RFC[1000]


Design note

The TEV cutting site used is the conventional site cleaved composed of 5 amino acids: ENLYFQ.

We have observed that this construction is weakly expressed. We advise future users to add a RiboJ insulator and a BCD2 element to improve its expression.

A double terminator controls the expression of this parts Part:BBa_B0015.

This sequence was synthesized by IDT DNA for iGEM Headquarters.