Difference between revisions of "Part:BBa K3147008:Design"
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A double terminator controls the expression ([[BBa_B0015]]). | A double terminator controls the expression ([[BBa_B0015]]). | ||
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The TEV protease used is a mutant TEV [1] with the following mutation: :T17A, T146A, D148P, S153C, S168T, S170A, T173A, S219P. | The TEV protease used is a mutant TEV [1] with the following mutation: :T17A, T146A, D148P, S153C, S168T, S170A, T173A, S219P. | ||
Revision as of 16:30, 20 October 2019
mutant TEV (PE10) protease with TEV-cleavable maltose binding protein
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 77
Illegal SapI.rc site found at 1868
A double terminator controls the expression (BBa_B0015).
The TEV protease used is a mutant TEV [1] with the following mutation: :T17A, T146A, D148P, S153C, S168T, S170A, T173A, S219P.
Reference
[1] Yi, L. et al. 2013. « Engineering of TEV Protease Variants by Yeast ER Sequestration Screening (YESS) of Combinatorial Libraries ». Proceedings of the National Academy of Sciences 110(18): 7229‑34