Difference between revisions of "Part:BBa K3147006:Design"
 
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<partinfo>BBa_K3147006 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K3147006 SequenceAndFeatures</partinfo>
  
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A double terminator controls the expression ([[BBa_B0015]]).
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A double terminator controls the expression ([[BBa_B0015]]).
  
 
The TEV protease used is a mutant TEV [1] with the following mutation: S120R, D148R, T173A, N177K, M218I, S219P.
 
The TEV protease used is a mutant TEV [1] with the following mutation: S120R, D148R, T173A, N177K, M218I, S219P.

Latest revision as of 16:26, 20 October 2019

mutant TEV (PE10) protease with TEV-cleavable maltose binding protein


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 77
    Illegal SapI.rc site found at 1868

A double terminator controls the expression (BBa_B0015).

The TEV protease used is a mutant TEV [1] with the following mutation: S120R, D148R, T173A, N177K, M218I, S219P.

Reference

[1] Yi, L. et al. 2013. « Engineering of TEV Protease Variants by Yeast ER Sequestration Screening (YESS) of Combinatorial Libraries ». Proceedings of the National Academy of Sciences 110(18): 7229‑34.