Difference between revisions of "Part:BBa K3147005:Design"
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The TEV protease is a highly specific cysteine protease of the tabacco ech virus sequence.
 
The TEV protease is a highly specific cysteine protease of the tabacco ech virus sequence.
−
The protease is highly specific for the consensus sequence for its cleavage site which is ENLYFQ\G (BBa_ J18918) with \ indicating cleaved peptide binding.
+
 
−
The Terminator used is a double terminator (BBa_0015).
+
The protease is highly specific for the consensus sequence for its cleavage site which is ENLYFQ\G ([[BBa_ J18918]]) with \ indicating cleaved peptide binding.
 +
 
 +
The Terminator used is a double terminator ([[BBa_0015]]).

Revision as of 16:24, 20 October 2019

TEV protease with TEV-cleavable maltose binding protein


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 379
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Note

The TEV protease is a highly specific cysteine protease of the tabacco ech virus sequence.

The protease is highly specific for the consensus sequence for its cleavage site which is ENLYFQ\G (BBa_ J18918) with \ indicating cleaved peptide binding.

The Terminator used is a double terminator (BBa_0015).