Difference between revisions of "Part:BBa K3059635"

 
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<partinfo>BBa_K3059635 short</partinfo>
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Synthetic curli operon under medium-weak constitutive promoter J23107. Curli fiber expression was assessed via Congo Red spin-down assays, where any present fibers bind Congo Red dye and hold it in the cell pellet, resulting in a lighter (less red) supernatant. Congo Red results are quantified by measuring A490 of this supernatant.
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For this circuit (WM19_024 or "circuit 24"), so visible pellets formed in the glass culture tubes during overnight growth, and no difference in redness was observed between control and experimental pellets after Congo Red staining. Furthermore, the average A490 measurement for experimental samples’ supernatants was slightly higher than the average for control circuits (if curli fibers were expressed, the supernatant should be lighter, resulting in a lower A490 measurement). Future constitutive circuits may require a stronger promoter such as J23100.
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More detailed characterization is available under the experience tab and on our wiki.
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===Usage and Biology===
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<span class='h3bb'>Sequence and Features</span>
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<partinfo>BBa_K3059635 SequenceAndFeatures</partinfo>
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===Functional Parameters===
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<partinfo>BBa_K3059635 parameters</partinfo>
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Revision as of 16:10, 20 October 2019


Constitutive Curli Operon: J23107

Synthetic curli operon under medium-weak constitutive promoter J23107. Curli fiber expression was assessed via Congo Red spin-down assays, where any present fibers bind Congo Red dye and hold it in the cell pellet, resulting in a lighter (less red) supernatant. Congo Red results are quantified by measuring A490 of this supernatant.

For this circuit (WM19_024 or "circuit 24"), so visible pellets formed in the glass culture tubes during overnight growth, and no difference in redness was observed between control and experimental pellets after Congo Red staining. Furthermore, the average A490 measurement for experimental samples’ supernatants was slightly higher than the average for control circuits (if curli fibers were expressed, the supernatant should be lighter, resulting in a lower A490 measurement). Future constitutive circuits may require a stronger promoter such as J23100.


More detailed characterization is available under the experience tab and on our wiki.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1873
    Illegal PstI site found at 972
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1873
    Illegal NheI site found at 51
    Illegal NheI site found at 74
    Illegal PstI site found at 972
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1873
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1873
    Illegal PstI site found at 972
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1873
    Illegal PstI site found at 972
    Illegal AgeI site found at 2170
  • 1000
    COMPATIBLE WITH RFC[1000]