Difference between revisions of "Part:BBa K3286046"
 
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Monomeric Red fluorescent protein (mRFP) [[part:BBa_K3286045]] and Green fluorescent protein (GFP) [[BBa_E0040]] downstream of bacteriophage lambda PL, PRM and PR promoter regions (including the operators OL and OR, and the 5'UTR's) [[part:BBa_K3286045]].  
 
Monomeric Red fluorescent protein (mRFP) [[part:BBa_K3286045]] and Green fluorescent protein (GFP) [[BBa_E0040]] downstream of bacteriophage lambda PL, PRM and PR promoter regions (including the operators OL and OR, and the 5'UTR's) [[part:BBa_K3286045]].  
 
This part has been used to asses the potential of bacteriophage lambda inhibition by CRISPRi, by targeting essential early promoters.  
 
This part has been used to asses the potential of bacteriophage lambda inhibition by CRISPRi, by targeting essential early promoters.  
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<ul><p>Atsumi, S., & Little, J. W. (2004). Regulatory circuit design and evolution using phage λ. Genes and Development, 18(17), 2086–2094. https://doi.org/10.1101/gad.1226004</p>
 +
<p>Court, D. L., Oppenheim, A. B., & Adhya, S. L. (2007). A new look at bacteriophage lambda genetic networks. Journal of Bacteriology, 189(2), 298–304. </p>
 +
<p>Liu, X., Jiang, H., Gu, Z., & Roberts, J. W. (2013). High-resolution view of bacteriophage lambda gene expression by ribosome profiling. Proceedings of the National Academy of Sciences, 110(29), 11928–11933. https://doi.org/10.1073/pnas.1309739110</p>
 +
<p>Ptashne, M. (2004). A genetic switch : phage lambda revisited. In Cold Spring Harbor Laboratory Press Cold Spring Harbor NY (Vol. 3).</p>
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<p>Wilson, H. R., Kameyama, L., Zhou, J. G., Guarneros, G., & Court, D. L. (1997). Translational repression by a transcriptional elongation factor. Genes and Development, 11(17), 2204–2213. https://doi.org/10.1101/gad.11.17.2204</p>
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</ul>
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<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Latest revision as of 16:06, 20 October 2019


mRFP-GFP divergent, Bacteriophage Lambda promoters, operators and 5'UTR's

Monomeric Red fluorescent protein (mRFP) part:BBa_K3286045 and Green fluorescent protein (GFP) BBa_E0040 downstream of bacteriophage lambda PL, PRM and PR promoter regions (including the operators OL and OR, and the 5'UTR's) part:BBa_K3286045. This part has been used to asses the potential of bacteriophage lambda inhibition by CRISPRi, by targeting essential early promoters.


    Atsumi, S., & Little, J. W. (2004). Regulatory circuit design and evolution using phage λ. Genes and Development, 18(17), 2086–2094. https://doi.org/10.1101/gad.1226004

    Court, D. L., Oppenheim, A. B., & Adhya, S. L. (2007). A new look at bacteriophage lambda genetic networks. Journal of Bacteriology, 189(2), 298–304.

    Liu, X., Jiang, H., Gu, Z., & Roberts, J. W. (2013). High-resolution view of bacteriophage lambda gene expression by ribosome profiling. Proceedings of the National Academy of Sciences, 110(29), 11928–11933. https://doi.org/10.1073/pnas.1309739110

    Ptashne, M. (2004). A genetic switch : phage lambda revisited. In Cold Spring Harbor Laboratory Press Cold Spring Harbor NY (Vol. 3).

    Wilson, H. R., Kameyama, L., Zhou, J. G., Guarneros, G., & Court, D. L. (1997). Translational repression by a transcriptional elongation factor. Genes and Development, 11(17), 2204–2213. https://doi.org/10.1101/gad.11.17.2204


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 7
    Illegal AgeI site found at 119
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1723