Difference between revisions of "Part:BBa K3286046"

Revision as of 16:05, 20 October 2019

mRFP-GFP divergent, Bacteriophage Lambda promoters, operators and 5'UTR's

Monomeric Red fluorescent protein (mRFP) part:BBa_K3286045 and Green fluorescent protein (GFP) BBa_E0040 downstream of bacteriophage lambda PL, PRM and PR promoter regions (including the operators OL and OR, and the 5'UTR's) part:BBa_K3286045. This part has been used to asses the potential of bacteriophage lambda inhibition by CRISPRi, by targeting essential early promoters.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal AgeI site found at 7
Illegal AgeI site found at 119
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 1723

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K3286046 short</partinfo>
-Monomeric Red fluorescent protein (mRFP) and Green fluorescent protein (GFP) downstream of bacteriophage lambda PL, PRM and PR promoter regions (including the operators OL and OR, and the 5'UTR's).
+Monomeric Red fluorescent protein (mRFP) [[part:BBa_K3286045]] and Green fluorescent protein (GFP) [[BBa_E0040]] downstream of bacteriophage lambda PL, PRM and PR promoter regions (including the operators OL and OR, and the 5'UTR's) [[part:BBa_K3286045]].
 This part has been used to asses the potential of bacteriophage lambda inhibition by CRISPRi, by targeting essential early promoters.