Difference between revisions of "Part:BBa K3286011"
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For both sgRNAs an rho-independent terminator is used. One sgRNA is under control of the strong constitutive Anderson promoter J23109, the other under the also constitutive Tac promoter.  
 
For both sgRNAs an rho-independent terminator is used. One sgRNA is under control of the strong constitutive Anderson promoter J23109, the other under the also constitutive Tac promoter.  
  
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<p>Anderson promoter J23109: https://parts.igem.org/Part:BBa_J23119</p>
+
<p>Anderson promoter J23109: [[Part:BBa_J23119]]</p>
−
<p>Tac promoter: https://parts.igem.org/Part:BBa_K2933026</p>
+
<p>Tac promoter: [[Part:BBa_K2933026]]</p>
−
<p>sgRNA expression cassette: https://parts.igem.org/Part:BBa_K3286001</p>
+
<p>sgRNA expression cassette: [[Part:BBa_K3286001]]</p>
−
<p>rho-independent terminator: https://parts.igem.org/Part:BBa_K3286002</p>
+
<p>rho-independent terminator: [[Part:BBa_K3286002]]</p>
  
  

Revision as of 15:22, 20 October 2019


sgRNA expression cassette (double target)

Double expression cassette of the sgRNA for Cas9 of Streptococcus pyogenes with non-targeting (NT) spacer.

For both sgRNAs an rho-independent terminator is used. One sgRNA is under control of the strong constitutive Anderson promoter J23109, the other under the also constitutive Tac promoter.

Anderson promoter J23109: Part:BBa_J23119

Tac promoter: Part:BBa_K2933026

sgRNA expression cassette: Part:BBa_K3286001

rho-independent terminator: Part:BBa_K3286002


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 197
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 197
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 197
  • 1000
    COMPATIBLE WITH RFC[1000]