Difference between revisions of "Part:BBa K3286009"

 
 
Line 5: Line 5:
 
The AcrIIA4 is under control of the inducible L-rhamnose promoter. The AcrIIA4 expression was adapted by the use of different RBS sites.  
 
The AcrIIA4 is under control of the inducible L-rhamnose promoter. The AcrIIA4 expression was adapted by the use of different RBS sites.  
  
−
The deeper analysis on varying Acr levels on inhibitory activity of dCas9 were assessed here: https://parts.igem.org/Part:BBa_K3286010
+
The deeper analysis on varying Acr levels on inhibitory activity of dCas9 were assessed here: [[Part:BBa_K3286010]]
  
−
L-rhamnose inducible promoter: https://parts.igem.org/Part:BBa_K914003
+
L-rhamnose inducible promoter: [[Part:BBa_K914003]]
  
−
AcrIIA4: https://parts.igem.org/Part:BBa_K3286000
+
AcrIIA4: [[Part:BBa_K3286000]]
  
−
bi-directional terminator: https://parts.igem.org/Part:BBa_K3286006
+
bi-directional terminator: [[Part:BBa_K3286006]]
  
  

Latest revision as of 15:16, 20 October 2019


AcrIIA4 expression cassette

The AcrIIA4 is under control of the inducible L-rhamnose promoter. The AcrIIA4 expression was adapted by the use of different RBS sites.

The deeper analysis on varying Acr levels on inhibitory activity of dCas9 were assessed here: Part:BBa_K3286010

L-rhamnose inducible promoter: Part:BBa_K914003

AcrIIA4: Part:BBa_K3286000

bi-directional terminator: Part:BBa_K3286006


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]