Difference between revisions of "Part:BBa K2984006:Design"

 
(→‎Source)
 
Line 13: Line 13:
 
===Source===
 
===Source===
  
−
Sizova, I., Fuhrmann, M., & Hegemann, P. (2001). A Streptomyces rimosusaphVIII gene coding for a new type phosphotransferase provides stable antibiotic resistance to Chla
+
This unpublished part was kindly provided by Irina Sizova of the Hegemann Lab at the Humboldt University of Berlin.
  
 
===References===
 
===References===

Latest revision as of 15:14, 20 October 2019


Codon-Usage-Optimized Paromomycin Resistance For Use in Chlamydomonas reinhardtii


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 298
    Illegal NotI site found at 122
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 13
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 401
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The codon usage optimization had to be adjusted to Chlamydomonas reinhardtii.


Source

This unpublished part was kindly provided by Irina Sizova of the Hegemann Lab at the Humboldt University of Berlin.

References