Difference between revisions of "Part:BBa K2924006"
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===Usage and Biology=== | ===Usage and Biology=== | ||
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+ | [[File:Thiolase_gene_location.png|thumb|right|400px|<i><b>Fig. 1:</b> Position of sgRNA (orange) in the beta ketoacyl-acyl carrier protein synthase gene.</i>]] | ||
+ | [[File:Thiolase_reaction.png|thumb|right|400px|<i><b>Fig. 2:</b> Reaction catalyzed by ketoacyl-acyl carrier protein synthase.</i>]] | ||
+ | https://parts.igem.org/File:Thiolase_gene_location.png | ||
+ | https://parts.igem.org/File:Thiolase_reaction.png | ||
+ | https://parts.igem.org/File:Knock_in_Schema.png | ||
+ | https://parts.igem.org/File:CRISPR_dCas9.png | ||
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+ | [[File:Knock_in_Schema.png|thumb|410px|left|<i><b>Fig. 3:</b> Scheme of a knock-in as a consequence of homologous recombination in Synechocystis.</i>]] | ||
+ | [[File:CRISPR_dCas9.png|thumb|right|450px|<i><b>Fig. 4:</b> Scheme of function of the CRISPRi/dCas9 - system. The dCas9 (yellow) binds with the sgRNA to the complementary DNA strand and inhibits the transcription by RNA polymerase II (blue).</i>]] | ||
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Revision as of 14:40, 20 October 2019
guideRNA from acetyl coenzyme A acetyltransferase
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Usage and Biology
https://parts.igem.org/File:Thiolase_gene_location.png https://parts.igem.org/File:Thiolase_reaction.png https://parts.igem.org/File:Knock_in_Schema.png https://parts.igem.org/File:CRISPR_dCas9.png
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]