Upon translation of the ribozyme into RNA, it cleaves itself off, removing any transcript from the mRNA upstream of the ribosomal binding site (RBS). This leads to a more stable expression independent of the cellular context <a href="http://2016.igem.org/Team:William_and_Mary/RiboJ">(See iGEM William and Mary 2016)</a> and can be used for better cross comparison of results over species border or the construction of more robust, species independent functional devices.
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Upon translation of the ribozyme into RNA, it cleaves itself off, removing any transcript from the mRNA upstream of the ribosomal binding site (RBS).
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<div style="width:100%;display:flex;flex-direction:row;flex-wrap: wrap; justify-content:space-evenly; align-items:center;">[[File:GE riboinsulators.png|500px|thumb|left|'''Figure 1''': Maturation of mRNA transcripts leads to a more standardized genetic context. ]]
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This leads to a more stable expression independent of the cellular context <a href="http://2016.igem.org/Team:William_and_Mary/RiboJ">(See iGEM William and Mary 2016)</a> and can be used for better cross comparison of results over species border or the construction of more robust, species independent functional devices.
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Revision as of 14:33, 20 October 2019
pMC_0_3_12_riboJ_B0034
Sequence and Features
Assembly Compatibility:
10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]
Description
This part contains the ribosomal binding site B0034 and riboJ which is a ribozyme.
Upon translation of the ribozyme into RNA, it cleaves itself off, removing any transcript from the mRNA upstream of the ribosomal binding site (RBS).
Figure 1: Maturation of mRNA transcripts leads to a more standardized genetic context.
This leads to a more stable expression independent of the cellular context (See iGEM William and Mary 2016) and can be used for better cross comparison of results over species border or the construction of more robust, species independent functional devices.
Compability
These parts are compatible with the RCF [1000] standard. These parts are compatible with any Part Collection that uses the Phytobrick standard of overhangs. For more information we recommend to head over to Design of the Marburg Collection.
