Difference between revisions of "Part:BBa K3093002:Design"
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===Design Notes=== | ===Design Notes=== | ||
| − | The sequence of Pcel and chbR was obtained from E.coli K12 MG1655 via PCR.Then we performed inverse PCR to achieve the site-directed mutation of amino acids of chbR. | + | The sequence of Pcel and chbR was obtained from E.coli K12 MG1655 via PCR.Then we performed inverse PCR to achieve the site-directed mutation of amino acids of chbR. |
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===Source=== | ===Source=== | ||
Latest revision as of 14:19, 20 October 2019
Cellobiose operon
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
The sequence of Pcel and chbR was obtained from E.coli K12 MG1655 via PCR.Then we performed inverse PCR to achieve the site-directed mutation of amino acids of chbR.
Source
E.coli K12 MG1655