Difference between revisions of "Part:BBa K2912014"

Latest revision as of 14:12, 20 October 2019

Tryptophan attenuator

Trp attenuator can act as a suicide switch controlled by the concentration of Tryptophan. Our team this year added this attenuator to the lysis gene as well as the R-body. The preamble sequence can translate a short peptide of 14 amino acids, in which there are two tryptophan codons at the 10th and 11th positions, followed by a sequence of RNA. The sequence can be divided into four segments, which can complement each other and form different secondary structures. Prokaryotes translate as they transcribe. The position of ribosome in the leading sequence determines which secondary structure is formed, which determines whether the attenuator can form a termination signal. (1) When tryptophan is present, the complete translated short peptide ribosome stays at the termination codon, and 2,3 pairs of adjacent segments hinder 2,3 pairs so that 3,4 pairs form hairpin terminator RNA enzymes terminate at the attenuator and cannot move forward. (2) In the absence of tryptophan, the ribosome arrives at the tryptophan codon without the supply of tryptophan tRNA and stays at the codon position. Located in Section 1, the RNA polymerase of Section 2 and Section 3 paired with Section 4 in a single-stranded state continues to move forward through the attenuator and transcribes the reverse complement sequence. For more information. click BBa_K2912015.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

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 <partinfo>BBa_K2912014 short</partinfo>
-Trp attenuator can act as a suicide switch controlled by the concentration of Tryptophan. Our team this year added this attenuator to the lysis gene as well as the R-body. The preamble sequence can translate a short peptide of 14 amino acids, in which there are two tryptophan codons at the 10th and 11th positions, followed by a sequence of RNA. The sequence can be divided into four segments, which can complement each other and form different secondary structures. Prokaryotes translate as they transcribe. The position of ribosome in the leading sequence determines which secondary structure is formed, which determines whether the attenuator can form a termination signal. (1) When tryptophan is present, the complete translated short peptide ribosome stays at the termination codon, and 2,3 pairs of adjacent segments hinder 2,3 pairs so that 3,4 pairs form hairpin terminator RNA enzymes terminate at the attenuator and cannot move forward. (2) In the absence of tryptophan, the ribosome arrives at the tryptophan codon without the supply of tryptophan tRNA and stays at the codon position. Located in Section 1, the RNA polymerase of Section 2 and Section 3 paired with Section 4 in a single-stranded state continues to move forward through the attenuator and transcribes the reverse complement sequence.
+Trp attenuator can act as a suicide switch controlled by the concentration of Tryptophan. Our team this year added this attenuator to the lysis gene as well as the R-body. The preamble sequence can translate a short peptide of 14 amino acids, in which there are two tryptophan codons at the 10th and 11th positions, followed by a sequence of RNA. The sequence can be divided into four segments, which can complement each other and form different secondary structures. Prokaryotes translate as they transcribe. The position of ribosome in the leading sequence determines which secondary structure is formed, which determines whether the attenuator can form a termination signal. (1) When tryptophan is present, the complete translated short peptide ribosome stays at the termination codon, and 2,3 pairs of adjacent segments hinder 2,3 pairs so that 3,4 pairs form hairpin terminator RNA enzymes terminate at the attenuator and cannot move forward. (2) In the absence of tryptophan, the ribosome arrives at the tryptophan codon without the supply of tryptophan tRNA and stays at the codon position. Located in Section 1, the RNA polymerase of Section 2 and Section 3 paired with Section 4 in a single-stranded state continues to move forward through the attenuator and transcribes the reverse complement sequence. For more information. click [https://parts.igem.org/Part:BBa_K2912015 BBa_K2912015.]
 <!-- Add more about the biology of this part here