Difference between revisions of "Part:BBa K2968002"

 
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<partinfo>BBa_K2968002 parameters</partinfo>
 
<partinfo>BBa_K2968002 parameters</partinfo>
 
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===KCL iGEM 2019===
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BBa_K2968002
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To generate functional sRNA molecule with the secondary structure shown in figure 1 our team has created this composite part consisting of three basic parts: BBa_J23100 promoter, BBa_K2968014 mRNA target binding region and BBa_K2968012 GcvB sRNA scaffold as shown in figure 2.
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https://2019.igem.org/wiki/images/5/55/T--KCL_UK--validation1a.png
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Figure 1. BBa_K2968002 expressing sRNA secondary structure predicted using http://rna.tbi.univie.ac.at/cgi-bin/RNAWebSuite/RNAfold.cgi server. The mRNA target binding region is highlighted in pink and the sRNA scaffold in teal. 
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https://2019.igem.org/wiki/images/4/49/T--KCL_UK--validation1b.png
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Figure 2. Details of the BBa_K2968002 sRNA construct design
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The BBa_J23100 promoter is a strong promoter from the constitutive promoter family designed by John Anderson, iGEM2006_Berkeley. BBa_K2968014 part is a complementary part to the BBa_K608010 that has BBa_B0034 Ribosome Binding Site (RBS) and BBa_E0040 GFP gene part as shown in figure 3. The GcvB scaffold is the 84 bp region of the E.coli sRNA.
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Revision as of 13:33, 20 October 2019


sRNA GcvB targeting BBa_B0034 and BBa_E0040 start codon

This sRNA has promoter target binding region for BBa_B0034 and BBa_E0040 start codon

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


KCL iGEM 2019

BBa_K2968002

To generate functional sRNA molecule with the secondary structure shown in figure 1 our team has created this composite part consisting of three basic parts: BBa_J23100 promoter, BBa_K2968014 mRNA target binding region and BBa_K2968012 GcvB sRNA scaffold as shown in figure 2.

T--KCL_UK--validation1a.png

Figure 1. BBa_K2968002 expressing sRNA secondary structure predicted using http://rna.tbi.univie.ac.at/cgi-bin/RNAWebSuite/RNAfold.cgi server. The mRNA target binding region is highlighted in pink and the sRNA scaffold in teal.

T--KCL_UK--validation1b.png

Figure 2. Details of the BBa_K2968002 sRNA construct design

The BBa_J23100 promoter is a strong promoter from the constitutive promoter family designed by John Anderson, iGEM2006_Berkeley. BBa_K2968014 part is a complementary part to the BBa_K608010 that has BBa_B0034 Ribosome Binding Site (RBS) and BBa_E0040 GFP gene part as shown in figure 3. The GcvB scaffold is the 84 bp region of the E.coli sRNA.