Difference between revisions of "Part:BBa K2922044:Design"
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__NOTOC__
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<partinfo>BBa_K2922044 short</partinfo>
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<partinfo>BBa_K2922044 SequenceAndFeatures</partinfo>
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===Design notes===
 
===Design notes===
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Construct from existed parts according to Standard BioBrick Assembly
 
Construct from existed parts according to Standard BioBrick Assembly
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===Source===
 
===Source===
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Cav1 is from homo sapiens. DsbA comes from Escherichia coli (Strain K12) and PbBD is a putative Pb2+ binding domain derived from PbrR.
 
Cav1 is from homo sapiens. DsbA comes from Escherichia coli (Strain K12) and PbBD is a putative Pb2+ binding domain derived from PbrR.

Revision as of 12:56, 20 October 2019


Co-expression system of BBa_K346030 and BBa_K2922042 for enhancing lead absorption


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 520
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 520
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 308
    Illegal XhoI site found at 1005
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 520
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 520
    Illegal AgeI site found at 140
  • 1000
    COMPATIBLE WITH RFC[1000]


Design notes

Construct from existed parts according to Standard BioBrick Assembly


Source

Cav1 is from homo sapiens. DsbA comes from Escherichia coli (Strain K12) and PbBD is a putative Pb2+ binding domain derived from PbrR.