Difference between revisions of "Part:BBa K2922043:Design"
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__NOTOC__
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<partinfo>BBa_K2922043 short</partinfo>
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<partinfo>BBa_K2922021 SequenceAndFeatures</partinfo>
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===Design notes===
 
===Design notes===
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We ordered the part DNA from Sangon ® company.
 
We ordered the part DNA from Sangon ® company.
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===Source===
 
===Source===
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DsbA comes from Escherichia coli (Strain K12) and PbBD is a putative Pb2+ binding domain derived from PbrR.
 
DsbA comes from Escherichia coli (Strain K12) and PbBD is a putative Pb2+ binding domain derived from PbrR.

Revision as of 12:55, 20 October 2019


Expression system of DsbA-PbBD coding periplasm display of lead binding peptide with T7 promoter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal NotI site found at 328
    Illegal NotI site found at 1472
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1385
    Illegal BamHI site found at 521
    Illegal XhoI site found at 883
    Illegal XhoI site found at 1132
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 663
    Illegal NgoMIV site found at 1588
    Illegal NgoMIV site found at 2906
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 567


Design notes

We ordered the part DNA from Sangon ® company.


Source

DsbA comes from Escherichia coli (Strain K12) and PbBD is a putative Pb2+ binding domain derived from PbrR.