Difference between revisions of "Part:BBa K2978201"

Latest revision as of 12:37, 20 October 2019

Control for Terminators Measuarement in Lactobacillus spp

This part is the negative control for the efficiency terminators assay, where we tested three different sequences for Lactobacillus spp K2978201 K2978202 K2978203 K2978204 and K2978205.
The composite part is based in constitutively express a GFP before of each terminator tested (in this specific part there is not terminator), and a RFP in a polycistronic way after the terminator. By comparing the fluorescence of each protein, the efficiency of the terminator could be determined.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K2978201 short</partinfo>
-This part is the negative control for the efficiency terminators assay, where we tested three different sequences for <Lactobacillus spp</i> [[Part:BBa_K2978201| K2978201]] [[Part:BBa_K2978202| K2978202]] [[Part:BBa_K2978203| K2978203]] [[Part:BBa_K2978204| K2978204]] and [[Part:BBa_K2978205| K2978205]].
+This part is the negative control for the efficiency terminators assay, where we tested three different sequences for <i>Lactobacillus spp</i> [[Part:BBa_K2978201| K2978201]] [[Part:BBa_K2978202| K2978202]] [[Part:BBa_K2978203| K2978203]] [[Part:BBa_K2978204| K2978204]] and [[Part:BBa_K2978205| K2978205]].
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 The composite part is based in constitutively express a GFP before of each terminator tested (in this specific part there is not terminator), and a RFP in a polycistronic way after the terminator. By comparing the fluorescence of each protein, the efficiency of the terminator could be determined.