Difference between revisions of "Part:BBa K2970002"

Revision as of 12:24, 20 October 2019

Gate

This gate is a toehold switch system with which a gene of interest can be locked and regulated on a translational level using mRNA as regulator. After transcription, the mRNA of this gate forms a hairpin that hides the ribosome binding site and start codon of the gene of interest, thus translation can not occur. A complementary part to the gate (trigger) is needed to open the hairpin and release the ribosome binding site. In this case two triggers are needed that form a trigger complex to open the gate (BBa_K2970000 and BBa_K2970001). The affinity between the trigger complex and the gate is greater than that of the gate to itself (in the loop). A single trigger cannot open the gate because it has only half the required complementary sequence.

To transform this part into bacteria it should be put on a plasmid together with a promoter and a terminator. We used this part to regulate a chloramphenicol resistance gene that we put behind the gate (BBa_K2970006).

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

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 <partinfo>BBa_K2970002 short</partinfo>
-This gate is a toehold switch system with which a gene of interest can be locked and regulated on a translational level using mRNA as regulator. After transcription, the mRNA of this gate forms a hairpin that hides the ribosome binding site and start codon of the gene of interest, thus translation can not occur. A complementary part to the gate (trigger) is needed to open the hairpin and release the ribosome binding site. In this case two triggers are needed that form a trigger complex to open the gate (<partinfo>BBa_K2970000</partinfo> and <partinfo>BBa_K2970001</partinfo>). The affinity between the trigger complex and the gate is greater than that of the gate to itself (loop). A single trigger cannot open the gate because it has only half the required complementary sequence.
+This gate is a toehold switch system with which a gene of interest can be locked and regulated on a translational level using mRNA as regulator. After transcription, the mRNA of this gate forms a hairpin that hides the ribosome binding site and start codon of the gene of interest, thus translation can not occur. A complementary part to the gate (trigger) is needed to open the hairpin and release the ribosome binding site. In this case two triggers are needed that form a trigger complex to open the gate (<partinfo>BBa_K2970000</partinfo> and <partinfo>BBa_K2970001</partinfo>). The affinity between the trigger complex and the gate is greater than that of the gate to itself (in the loop). A single trigger cannot open the gate because it has only half the required complementary sequence.
 To transform this part into bacteria it should be put on a plasmid together with a promoter and a terminator. We used this part to regulate a chloramphenicol resistance gene that we put behind the gate (<partinfo>BBa_K2970006</partinfo>).