Difference between revisions of "Part:BBa I712004:Experience"

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===Usage and Biology===
 
===Usage and Biology===
 
We validated this part by insert it and eGFP together into the vertor. The map of the plasmid is as followed(Fig.1). Then we transfected the plasmid to 293T cells, the expression of eGFP protein was observed by fluorescence microscope(Fig.2). You can also see the characterization of this part in our composite part BBa_K2942705, BBa_K2942706, BBa_K2942707, BBa_K2942708.
 
We validated this part by insert it and eGFP together into the vertor. The map of the plasmid is as followed(Fig.1). Then we transfected the plasmid to 293T cells, the expression of eGFP protein was observed by fluorescence microscope(Fig.2). You can also see the characterization of this part in our composite part BBa_K2942705, BBa_K2942706, BBa_K2942707, BBa_K2942708.
[[File:2004_figure1.jpg]]Fig.1 The plasmid we used for transfection.
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[[File:2004_figure1.jpg|720px]]
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Fig.1 The plasmid we used for transfection.
  
 
Fig.2 Transfection cells observed under a fluorescence microscope
 
Fig.2 Transfection cells observed under a fluorescence microscope

Revision as of 12:21, 20 October 2019


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Applications of BBa_I712004

Usage and Biology

We validated this part by insert it and eGFP together into the vertor. The map of the plasmid is as followed(Fig.1). Then we transfected the plasmid to 293T cells, the expression of eGFP protein was observed by fluorescence microscope(Fig.2). You can also see the characterization of this part in our composite part BBa_K2942705, BBa_K2942706, BBa_K2942707, BBa_K2942708. 2004 figure1.jpg

Fig.1 The plasmid we used for transfection.

Fig.2 Transfection cells observed under a fluorescence microscope

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