Difference between revisions of "Part:BBa K3196036"
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<partinfo>BBa_K3196036 short</partinfo> | <partinfo>BBa_K3196036 short</partinfo> | ||
| + | <h1>'''Characterization'''</h1> | ||
It is a strong promoter induced by methanol and is often used to produce recombinant protein in Pichia pastoris expression system. | It is a strong promoter induced by methanol and is often used to produce recombinant protein in Pichia pastoris expression system. | ||
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| + | <h1>'''Usage and Biology'''</h1> | ||
| + | AOX1 gene is the main source of alcohol oxidase (Aox). AOX1 promoter is strictly induced by methanol and inhibited in the presence of glucose, glycerol, fructose and other carbon sources. | ||
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Revision as of 12:21, 20 October 2019
AOX1
Characterization
It is a strong promoter induced by methanol and is often used to produce recombinant protein in Pichia pastoris expression system.
Usage and Biology
AOX1 gene is the main source of alcohol oxidase (Aox). AOX1 promoter is strictly induced by methanol and inhibited in the presence of glucose, glycerol, fructose and other carbon sources.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]