Difference between revisions of "Part:BBa K2978203"

Revision as of 12:16, 20 October 2019

Terminator 667 Measurement in Lactobacillus spp

This part is a sample for the efficiency terminators assay, where we tested three different sequences for <Lactobacillus spp</i> K2978201 K2978202 K2978203 K2978204 and K2978205.

The composite part is based in constitutively express a GFP before of each terminator tested, and a RFP in a polycistronic way after the terminator. By comparing the fluorescence of each protein, the efficiency of the terminator could be determined.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

Revision as of 11:56, 20 October 2019 (view source) Registry (Talk \| contribs)		Revision as of 12:16, 20 October 2019 (view source) PabloD CR (Talk \| contribs) Newer edit →
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	This part is a sample for the efficiency terminators assay, where we tested three different sequences for <Lactobacillus spp</i> [[Part:BBa_K2978201\| K2978201]] [[Part:BBa_K2978202\| K2978202]] [[Part:BBa_K2978203\| K2978203]] [[Part:BBa_K2978204\| K2978204]] and [[Part:BBa_K2978205\| K2978205]].		This part is a sample for the efficiency terminators assay, where we tested three different sequences for <Lactobacillus spp</i> [[Part:BBa_K2978201\| K2978201]] [[Part:BBa_K2978202\| K2978202]] [[Part:BBa_K2978203\| K2978203]] [[Part:BBa_K2978204\| K2978204]] and [[Part:BBa_K2978205\| K2978205]].

−	The composite part is based in constitutively express a GFP before of each terminator tested ~~(in this specific part there is not terminator)~~, and a RFP in a polycistronic way after the terminator. By comparing the fluorescence of each protein, the efficiency of the terminator could be determined.	+	The composite part is based in constitutively express a GFP before of each terminator tested, and a RFP in a polycistronic way after the terminator. By comparing the fluorescence of each protein, the efficiency of the terminator could be determined.