Difference between revisions of "Part:BBa K3058000"
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This part encode a uricase (from Cyberlindnera jadinii) under the control of the constitutive promoter J23100 and strong RBS B0034. The uricase encoded can covert uric acid into hydroxyisourate. In our project, we express it in E. coli. It is hoped that the uricase-expressing E.coli can lower the uric acid concentration in human alimentary canal, so as to prevent gout disease. | This part encode a uricase (from Cyberlindnera jadinii) under the control of the constitutive promoter J23100 and strong RBS B0034. The uricase encoded can covert uric acid into hydroxyisourate. In our project, we express it in E. coli. It is hoped that the uricase-expressing E.coli can lower the uric acid concentration in human alimentary canal, so as to prevent gout disease. | ||
| − | https://2019.igem.org/wiki/images/f/fc/T--Hong_Kong_LFC_PC--uridna.jpg | + | <center>"https://2019.igem.org/wiki/images/f/fc/T--Hong_Kong_LFC_PC--uridna.jpg" </center> |
https://2019.igem.org/wiki/images/0/04/T--Hong_Kong_LFC_PC--urisds.jpg | https://2019.igem.org/wiki/images/0/04/T--Hong_Kong_LFC_PC--urisds.jpg | ||
https://static.igem.org/mediawiki/parts/5/55/T--Hong_Kong_LFC_PC--Uricasecolor2.jpg | https://static.igem.org/mediawiki/parts/5/55/T--Hong_Kong_LFC_PC--Uricasecolor2.jpg | ||
https://static.igem.org/mediawiki/parts/9/9b/T--Hong_Kong_LFC_PC--Uricasegraph.jpg | https://static.igem.org/mediawiki/parts/9/9b/T--Hong_Kong_LFC_PC--Uricasegraph.jpg | ||
https://2019.igem.org/wiki/images/6/67/T--Hong_Kong_LFC_PC--UricaseTP.jpg | https://2019.igem.org/wiki/images/6/67/T--Hong_Kong_LFC_PC--UricaseTP.jpg | ||
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Latest revision as of 12:15, 20 October 2019
Uricase from Cyberlindnera jadinii
This part encode a uricase (from Cyberlindnera jadinii) under the control of the constitutive promoter J23100 and strong RBS B0034. The uricase encoded can covert uric acid into hydroxyisourate. In our project, we express it in E. coli. It is hoped that the uricase-expressing E.coli can lower the uric acid concentration in human alimentary canal, so as to prevent gout disease.
" 
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 510
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 532
- 1000COMPATIBLE WITH RFC[1000]