Difference between revisions of "Part:BBa K121012"
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Using iGEM Tokyo_Tech assay protocol, we identified hill coefficient of Plac_GFP in BBa_I739003 in ''E. Coli'' strain JM109.
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We identified hill coefficient of Plac_GFP in BBa_I739003 in ''E. Coli'' strain JM109.
  
 
[[Image:Transfer Function K121012.png]]
 
[[Image:Transfer Function K121012.png]]

Revision as of 15:12, 22 October 2008

Plac GFP on pSB6

pressure and IPTG inducible Plac GFP reporter

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 267
    Illegal BamHI site found at 255
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 954

Usage and Biology

Identify Hill coefficient by IPTG induction

We identified hill coefficient of Plac_GFP in BBa_I739003 in E. Coli strain JM109.

Transfer Function K121012.png

The hill coefficient is 2.15.

Pressure induction