Difference between revisions of "Part:BBa K121012"
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pressure and IPTG inducible Plac GFP reporter | pressure and IPTG inducible Plac GFP reporter | ||
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===Usage and Biology=== | ===Usage and Biology=== | ||
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Revision as of 15:11, 22 October 2008
Plac GFP on pSB6
pressure and IPTG inducible Plac GFP reporter
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 267
Illegal BamHI site found at 255 - 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 954
Usage and Biology
Identify Hill coefficient by IPTG induction
Using iGEM Tokyo_Tech assay protocol, we identified hill coefficient of Plac_GFP in BBa_I739003 in E. Coli strain JM109.
The hill coefficient is 2.15.
Pressure induction