Difference between revisions of "Part:BBa K2904015"

 
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<partinfo>BBa_K2904015 short</partinfo>
 
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==<strong>Design</strong>==
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===Background of 2019 OUC-China's project——RiboLego===
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<p>
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Due to context-dependent performance and limited dynamic range, the widespread application of riboswitches is currently restricted. By replacing its original ORF with a new one, the structure of an aptamer domain can be subtly disrupted, resulting in a loss of ligand response. So riboswitch is still not be considered as a ‘plug and play' device. To tackle these problems, our project focuses on a standardized design principle to be used for modular and  tunable riboswitch. The modular riboswitch we defined consists of the original riboswitch, Stabilizer and Tuner. Stabilizer can protect the structure of riboswitch from damage while Tuner can reduce the expression probability of fusion protein and make improvement of riboswitch function.
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</p>
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===The construction of this part===
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When it comes to Stabilizer, a necessary factor to consider is the source. In addition to blast, by which the original target gene is selected from the genome, we can also choose the gene working well under the riboswitch from the past study. To validate the soure of Stabilizer is changable, we employed the first 150 bases of GFP as Stabilizer of Adda riboswitch because it can regulate the expression of GFP normally. This part was modular Adda riboswitch containing the original riboswitch, Stabilizer and Tuner B.
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Modular Adda riboswitch containing a different source of Stabilizer and Tuner A
 
  
 
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Revision as of 11:45, 20 October 2019


Modular Adda riboswitch containing a different source of Stabilizer and Tuner B

Design

Background of 2019 OUC-China's project——RiboLego

Due to context-dependent performance and limited dynamic range, the widespread application of riboswitches is currently restricted. By replacing its original ORF with a new one, the structure of an aptamer domain can be subtly disrupted, resulting in a loss of ligand response. So riboswitch is still not be considered as a ‘plug and play' device. To tackle these problems, our project focuses on a standardized design principle to be used for modular and tunable riboswitch. The modular riboswitch we defined consists of the original riboswitch, Stabilizer and Tuner. Stabilizer can protect the structure of riboswitch from damage while Tuner can reduce the expression probability of fusion protein and make improvement of riboswitch function.

The construction of this part

When it comes to Stabilizer, a necessary factor to consider is the source. In addition to blast, by which the original target gene is selected from the genome, we can also choose the gene working well under the riboswitch from the past study. To validate the soure of Stabilizer is changable, we employed the first 150 bases of GFP as Stabilizer of Adda riboswitch because it can regulate the expression of GFP normally. This part was modular Adda riboswitch containing the original riboswitch, Stabilizer and Tuner B.



Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]