Difference between revisions of "Part:BBa K121012"
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| − | + | Using iGEM Tokyo_Tech assay protocol, we identified hill coefficient of Plac_GFP in BBa_I739003 in ''E. Coli'' strain JM109. | |
| − | + | [[Image:Transfer Function K121012.png]] | |
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| − | [[Image:Transfer Function K121012. | + | |
The hill coefficient is 2.15. | The hill coefficient is 2.15. | ||
Revision as of 14:40, 22 October 2008
Plac GFP on pSB6
pressure and IPTG inducible Plac GFP reporter
Usage and Biology
Identify Hill coefficient by IPTG induction
Using iGEM Tokyo_Tech assay protocol, we identified hill coefficient of Plac_GFP in BBa_I739003 in E. Coli strain JM109.
The hill coefficient is 2.15.
Pressure induction
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 267
Illegal BamHI site found at 255 - 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 954