Difference between revisions of "Part:BBa K3245015"

 
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<h2>Description:</h2>
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<p>This composite part is used for measurement of the new hybrid promoter. </p>
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<h2>Usage:</h2>
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<p>Transfer with high copy control plasmid containing luxI-luxR part ( BBa_K3245002 ) to test real time quorum sensing behavior and collect data such as OD600 and fluorescence.
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Transfer with plasmid containing luxR part ( BBa_C0062, etc. ) and start measuring with artificial AHL induction. It’s more suitable when accurate mechanism or dynamics research is needed.
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</p>
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<h2>Characterization:</h2>
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<p>Please refer to https://parts.igem.org/Part:BBa_K3245001 </p>
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__NOTOC__
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<partinfo>BBa_K3245014 short</partinfo>
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<!-- Add more about the biology of this part here
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===Usage and Biology===
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<!-- -->
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<span class='h3bb'>Sequence and Features</span>
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<partinfo>BBa_K3245014 SequenceAndFeatures</partinfo>
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<!-- Uncomment this to enable Functional Parameter display
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===Functional Parameters===
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<partinfo>BBa_K3245014 parameters</partinfo>
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<!-- -->
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__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_K3245015 short</partinfo>
 
<partinfo>BBa_K3245015 short</partinfo>
  
waiting
 
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 11:33, 20 October 2019

Description:

This composite part is used for measurement of the new hybrid promoter.

Usage:

Transfer with high copy control plasmid containing luxI-luxR part ( BBa_K3245002 ) to test real time quorum sensing behavior and collect data such as OD600 and fluorescence. Transfer with plasmid containing luxR part ( BBa_C0062, etc. ) and start measuring with artificial AHL induction. It’s more suitable when accurate mechanism or dynamics research is needed.

Characterization:

Please refer to https://parts.igem.org/Part:BBa_K3245001



This composite part is used for measurement of the new promoter.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 26
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]




This composite part is used for measurement of the new hybrid promoter.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 26
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]