Difference between revisions of "Part:BBa K3245015"
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+ | |||
+ | <h2>Description:</h2> | ||
+ | <p>This composite part is used for measurement of the new hybrid promoter. </p> | ||
+ | <h2>Usage:</h2> | ||
+ | <p>Transfer with high copy control plasmid containing luxI-luxR part ( BBa_K3245002 ) to test real time quorum sensing behavior and collect data such as OD600 and fluorescence. | ||
+ | Transfer with plasmid containing luxR part ( BBa_C0062, etc. ) and start measuring with artificial AHL induction. It’s more suitable when accurate mechanism or dynamics research is needed. | ||
+ | </p> | ||
+ | |||
+ | <h2>Characterization:</h2> | ||
+ | |||
+ | <p>Please refer to https://parts.igem.org/Part:BBa_K3245001 </p> | ||
+ | |||
+ | |||
+ | |||
+ | __NOTOC__ | ||
+ | <partinfo>BBa_K3245014 short</partinfo> | ||
+ | |||
+ | |||
+ | <!-- Add more about the biology of this part here | ||
+ | ===Usage and Biology=== | ||
+ | |||
+ | <!-- --> | ||
+ | <span class='h3bb'>Sequence and Features</span> | ||
+ | <partinfo>BBa_K3245014 SequenceAndFeatures</partinfo> | ||
+ | |||
+ | |||
+ | <!-- Uncomment this to enable Functional Parameter display | ||
+ | ===Functional Parameters=== | ||
+ | <partinfo>BBa_K3245014 parameters</partinfo> | ||
+ | <!-- --> | ||
+ | |||
+ | |||
__NOTOC__ | __NOTOC__ | ||
<partinfo>BBa_K3245015 short</partinfo> | <partinfo>BBa_K3245015 short</partinfo> | ||
− | |||
<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here |
Revision as of 11:33, 20 October 2019
Description:
This composite part is used for measurement of the new hybrid promoter.
Usage:
Transfer with high copy control plasmid containing luxI-luxR part ( BBa_K3245002 ) to test real time quorum sensing behavior and collect data such as OD600 and fluorescence. Transfer with plasmid containing luxR part ( BBa_C0062, etc. ) and start measuring with artificial AHL induction. It’s more suitable when accurate mechanism or dynamics research is needed.
Characterization:
Please refer to https://parts.igem.org/Part:BBa_K3245001
This composite part is used for measurement of the new promoter.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 26
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
This composite part is used for measurement of the new hybrid promoter.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 26
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]