Difference between revisions of "Part:BBa K2983053"
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It is an improvement of [[Part:BBa_K2117000|BBa_K2117000]] as it is shorter and does not have the BsaI site which makes this part compatible with the iGEM Type IIS RFC[1000] standard. | It is an improvement of [[Part:BBa_K2117000|BBa_K2117000]] as it is shorter and does not have the BsaI site which makes this part compatible with the iGEM Type IIS RFC[1000] standard. | ||
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+ | ===Usage and Biology=== | ||
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+ | TEF is an isolated promoteur with highly expressed genes in ''yarrowia lipolytica'' and present in only on copy in his genome. TEF encode for translation elongation factor-1 α. | ||
<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here |
Revision as of 11:32, 20 October 2019
pTef1f: wild-type without the BsaI site, shorter and with CACA before ATG
This part is a version of the Yarrowia lipolytica pTef1 promoter, derived from the wild type pTef1 promoter (nucleotides 1227367 to 1227009) of Yarrowia lipolytica W29 chromosome C (GenBank Acc. n° CP028450.1). Four additional nucleotides (CACA) are present at the end of this part in order to be directly upstream of the ATG of a gene placed under its control.
It is an improvement of BBa_K2117000 as it is shorter and does not have the BsaI site which makes this part compatible with the iGEM Type IIS RFC[1000] standard.
Usage and Biology
TEF is an isolated promoteur with highly expressed genes in yarrowia lipolytica and present in only on copy in his genome. TEF encode for translation elongation factor-1 α.
Sequence and Features
- 10INCOMPATIBLE WITH RFC[10]Illegal SpeI site found at 192
Illegal PstI site found at 157 - 12INCOMPATIBLE WITH RFC[12]Illegal SpeI site found at 192
Illegal PstI site found at 157 - 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal SpeI site found at 192
Illegal PstI site found at 157 - 25INCOMPATIBLE WITH RFC[25]Illegal SpeI site found at 192
Illegal PstI site found at 157 - 1000COMPATIBLE WITH RFC[1000]