Difference between revisions of "Part:BBa K2983052"

 
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It is an improvement of [[Part:BBa_K2117000|BBa_K2117000]] as it is shorter and does not have the BsaI site which makes this part compatible with the iGEM Type IIS RFC[1000] standard.
 
It is an improvement of [[Part:BBa_K2117000|BBa_K2117000]] as it is shorter and does not have the BsaI site which makes this part compatible with the iGEM Type IIS RFC[1000] standard.
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===Usage and Biology===
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TEF is an isolated promoteur  with highly expressed genes in ''yarrowia lipolytica'' and present in only on copy in his genome. TEF encode for translation elongation factor-1 α.
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 11:31, 20 October 2019


pTef1e: wild-type without the BsaI site and shorter

This part is a version of the Yarrowia lipolytica pTef1 promoter, derived from the Addgene plasmid #120738.

It is an improvement of BBa_K2117000 as it is shorter and does not have the BsaI site which makes this part compatible with the iGEM Type IIS RFC[1000] standard.

Usage and Biology

TEF is an isolated promoteur with highly expressed genes in yarrowia lipolytica and present in only on copy in his genome. TEF encode for translation elongation factor-1 α.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 192
    Illegal PstI site found at 157
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 192
    Illegal PstI site found at 157
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal SpeI site found at 192
    Illegal PstI site found at 157
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 192
    Illegal PstI site found at 157
  • 1000
    COMPATIBLE WITH RFC[1000]